Genemed CISH and FISH ProbesHOT!provide a practical method to detect gene aberration, including gene amplification, deletion, chromosome/gene copy number change, chromosome translocation, gene expression at RNA level, and viral/bacterial detection. With CISH, tissue and cell morphology can be evaluated simultaneously with the gene status by using bright-field microscopy and standard immunohistochemistry techniques (conventional peroxidase or alkaline phosphatase reactions). Genemed multiplex FISH allows for simultaneous detection of multiple targets using dual-colored probes.
Genemed offers Primary Antibodies with high affinity and specificity for in vitro diagnostic and research use. Our primary antibodies are optimized and titrated for immunohistochemistry (IHC) testing using human formalin-fixed, paraffin-embedded (FFPE) tissue sections along with the Genemed IHC Detection Systems and Ancillary reagents.
Genemed Primary Antibodies
Detection Systems
Genemed's sensitive and easy to use Detection Systems enable strong staining signals with lowest possible background. We provide ready-to-use IHC detection reagents to meet variable needs, e.g. for mouse, or/and rabbit primary antibody; peroxidase (HRP) or alkaline phosphatase (AP) enzymes.
The following table summarizes Genemed's Detection Systems.
PowerStain 1.0 Kit Poly HRP DAB
PowerStain 1.0 Kit Poly HRP AEC
PowerStain 2.0 Kit Poly HRP
Acu-Stain HRP kit
Steps
One
One
Two
Two
Mechanism
Non-biotin system, uses HRP to localize primary antibody bound to target antigen
Non-biotin system, uses HRP to localize primary antibody bound to target antigen
Non-biotin, but uses linker to localize primary antibody bound to target antigen
Biotinylated secondary antibody binds to Streptavidin-HRP and to primary antibody bound to target antigen
Chromogen
Sensitive DAB (brown)
Included
AEC (Red)
Included
HRP substrate, DAB suggested, but not included
HRP substrate, either DAB or AEC but not included
Benefit
Fast protocol
Fast protocol
Signal amplification
Signal amplification
Best value
Detection Incubation Time
15 min
15 min
15 min + 15 min
10 min + 10 min
Sensitivity
High sensitivity
High sensitivity
High sensitivity
High sensitivity
Primary Antibody Reactivity
Mouse and Rabbit
Mouse and Rabbit
Mouse and Rabbit
Mouse and Rabbit
Specimen
Human
Human
Human
Human
Ancillary Reagents
Genemed provides a variety of antigen retrieval reagents, substrates, and buffers to facilitate your staining process. This product line includes pretreatment buffers and enzymes, blocking solutions, diluents, chromogens, counterstains, mounting media, and general buffers commonly used in routine IHC testing. All Ancillary Reagents are optimized to enhance the performance of Genemed primary antibodies and detection systems.
